This article is about the OT-One.

It's no-longer sold or actively supported by Opentrons, but we've kept this article here to help existing users. Please see our OT-2 Support articles for the most up to date information!

  1. Protocol Upload Failure: API Deprecation 

Having trouble uploading your protocol to OT One app v.2.5.1? The API has gone through some significant changes. These changes does not affect what the API does but how it does them. Therefore, select commands no longer exist in the API in v.2.5.1

The following commands have been deprecated from the API since the commands are no longer enqueued:

In order to remove the enqueue, simply delete the command inside the parenthesis



If you have any further issues editing your protocol, our support team would be happy to help. Email us the protocol at or message us via live chat.

For a more detailed explanation of the changes that have been made with the release of Opentrons app and API, please visit the blog post on the Opentrons website.

You can download v.2.5.1 here

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