The Opentrons Magnetic Module is designed to automate magnetic bead based protocols on the OT-2. The magnetic module (sometimes referred to as the "magdeck") is compatible with most magnetic bead kits on the market, allowing biologists to automate nucleic acid purification protocols including PCR clean-up, genomic DNA extraction, plasmid purification, RNA extraction, and more.
This article goes through:
- Compatible labware
- Installing the magnetic module on the OT-2
- Creating a protocol using the magnetic module
- Calibrating a protocol using the magnetic module
- GEN1 vs GEN2
Installing the Magnetic Module on the OT-2
1. Place module on the deck.
The Magnetic Module fits directly onto slots on the OT-2s deck.
For guidance and recommendations on where to plate your Magnetic Module, read Module Placement on the OT-2
The Magnetic Module requires two different connections, one to the power source and one to the OT-2 for control by the robot. The pronged jack is the power cable and the USB Type-A goes into the USB port in the back of the robot.
The power cable connects directly to a large power supply brick that plugs into the wall power outlet. The control cable goes to the USB sockets in the top left corner of the OT-2
Use the cable holders on the side of the robot to keep the USB cable out of the way.
[CABLE HOLDER IMAGE FORTHCOMING]
3. Power On & Check Connection
Press the small black button to power-on the magnetic module. You should hear some small motor noises as the module homes itself on boot.
Now your robot should be able to see that it has a magdeck connected to it. Open the OT App and connect to your robot over USB or WiFi. Then you can click on the "Pipettes & Modules" tab below the name of your robot. You should see the pipettes and modules connected to your robot, as in the screenshot below:
Using your Magnetic Module in OT-2 Protocols
You can incorporate your Magnetic Module into your Python API or Protocol Designer protocols.
- Learn about using your Magnetic Module in Protocol Designer protocols here. Protocol Designer support for Modules was introduced in software version 3.17, in May 2020.
- Request a protocol from the Opentrons team using the Protocol Request Form
- You can also find some protocols using the magnetic module on our Protocol Library.
- Read on or go directly to our API documentation to learn about using a Magnetic Module in a Python API protocol
Writing a Python API Protocol for your Magnetic Module
There are a three things you need to include in your python protocol to be able to use the magnetic module.
- Include the modules package from the Opentrons library:
from opentrons import modules
2. Instantiate your magdeck in a deck slot (in this case, slot 1):
magdeck = modules.load('magdeck', '1')
3. Put a PCR plate on top of the magdeck:
plate = labware.load('96-PCR', '1', share = True)
Note the "share = True" that indicates this plate is on top of the magdeck, sharing the deck slot with it.
If you include those three things, you can feel free to use the magdeck in your protocol. You can transfer, distribute, etc into the plate on top of the magdeck. You can engage and disengage the magnets using
Calibrating a Protocol with the Magnetic Module
When you have completed your protocol using the magdeck, it is time to load it into the Opentrons App and run it.
The robot will be able to see that your protocol includes a MagDeck and will check to make sure that it is connected. It will then display the module in the deck slot the protocol you assigned to the MagDeck (Slot 1 in the example below). You can then proceed through labware calibration in the same way you normally would. The robot will move to the A1 location of the plate on top of the MagDeck for you to save the position in the same way as it does for every other labware.
Once you have completed your calibration, you can run your protocol!
GEN1 vs GEN2 Magnetic Modules
How to tell if which generation of module you have
GEN1 magnetic modules have long magnetic bars. The GEN2 modules have more, shorter, magnetic bars.
A GEN1 magnetic module (pictured left) and a GEN2 magnetic module (pictured right)
When switching between generations there are a couple things to keep in mind.
- There will be a difference in incubation time. The magnets on the GEN2 module are more gentle. Because of this if you are changing a protocol to use a GEN2 module instead of a GEN1 module you may need to increase the time you wait for the magnetic beads to settle into place. Please experiment with time to see what works best with your module and beads.
- There is a difference in how engage height is represented. The units for GEN1 module engage height were not based on a standard unit of measurement. Engage height for GEN2 modules is in millimeters.
Converting engage height between generations
- To convert engage height from GEN1 to GEN2, divide by 2.
- To convert engage height from GEN2 to GEN1, multiply by 2.
- If you are using the Protocol Designer, compatible labware, and have not changed any of the default engage heights in your protocol, this conversion will be done for you automatically when you switch generations.