Warning: Do not plug in or power on your robot. You will be prompted to do so at a later step in the Get Started Guide.


To prevent damage during shipping, the moving parts of your OT-2 were locked in place with 3 locking brackets.

You'll need to remove the brackets before turning on your OT-2 for the first time.

You will be able to easily identify the specific hardware: each screw and bracket is flagged with blue tape. The brackets themselves may be black or red.

Materials Needed

  • 3 mm hex key (included)


Turn your robot around so the blue flags on the locking hardware are most accessible to you.

1. The first two screws to remove can be found behind the pipette carriage. It can be accessed via the front opening door. Use your 3 mm hex key to remove these screws.

2. Collect the two square nuts that fell out of the pipette carriage.

If no nuts fell out, they might be stuck inside the channel. We recommend removing them now so there's no risk of them getting shaken loose and falling out during a protocol run.

To remove the stuck nuts:

  1. Use your 2 mm hex key to loosen the small screw inside the pipette enclosure, so the nuts have more room to slide past it.

  2. If the nuts still haven't fallen out, stick your hex key up the channel and knock them loose. They should fall out now.

  3. Retighten the small screw.

If you're having trouble removing the two square nuts, either leave them in or contact Opentrons Support for help.

3. The next set of screws are located slightly above the pipette carriage.  There are 3 screws in total. In order to access the third screw, first the 2 screws shown below must be removed.

Use your 3 mm hex key to remove these screws. 

4. Move z carriage over to allow better access to the third locking screw.

Then, unscrew the final locking screw to remove the bracket. 

5.  Now that the carriage is out of the way, the two screws for bottom locking bracket is accessible.

Using your 3 mm hex key, remove the screws. 

6. The last locking bracket is the smallest and easiest to remove. 

Using your 3 mm hex key, remove the screw and small L-shaped locking bracket.  

If you have any questions, please reach out to us at support@opentrons.com or through our live chat!

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