Before you can use your OT-2, you'll need to perform a deck calibration.
Deck calibration is one important part your OT-2's positional calibration, which is what makes sure it moves its pipettes to the right places. To learn more about how it all fits together, check out how positional calibration works on the OT-2.
What you'll need
- At least one pipette already attached to your OT-2.
- Opentrons tips for that pipette, in an Opentrons tip rack. (Third-party tips won't work.)
Part 1: Begin
On the Robot tab, make sure you're connected to your OT-2.
Find the Robot Calibration > Calibrate deck section. Select the Calibrate button.
You might see warnings that say "calibration required." If you do, you can ignore them, for now. They'll go away when you finish this procedure.
Part 2: Perform the deck calibration
Closely follow the Opentrons App's instructions to perform the deck calibration. It will guide you through:
- Loading an Opentrons tip rack.
- Having one of your pipettes pick up a tip.
- Lowering that pipette to the deck surface.
- Jogging that pipette to several reference points on the deck.
Part 3: Finish up
If all has gone well, the OT-2 will return the tip to its rack and save the deck calibration.
You should see a green check mark and the message "Deck calibration complete."
When to perform a deck calibration
You can repeat this process to perform a new deck calibration whenever you want, but you only need to do it once in a while. You should only need to do it:
- As part of setting the OT-2 up for the first time.
- After moving the OT-2 more than a short distance.
- Once or twice per year, for maintenance.
- When troubleshooting certain movement, positioning, and calibration problems.