Next, you will need to perform deck calibration on your OT-2. This step is required when you first set up your robot to ensure your deck is calibrated correctly. Our hardware team recommends re-doing deck calibration about once a year for maintenance.
- Mounted pipette
- Opentrons tips (required for deck calibration, otherwise you will get a poor calibration)
- Connect to your robot and select the calibrate button.
2. Remove all labware and tipracks from the deck.
3. Select "Move Pipette to the Front" to initiate deck calibration - your left pipette will now move to the front and lower itself.
4. Step 1 of deck calibration is to attach a tip on the descended pipette.
5. Select "Confirm Tip Attached".
6. Step 2 of deck calibration is to move the pipette with tip attached to be flush to the deck using the jog keys. This will save data for the z-axis. You will be given the option to change the jump size of each jog command (0.1 mm, 1 mm, and 10 mm).
7. Select "Save Calibration and Continue" once successfully flushed with the deck.
8. Step 3 of deck calibration is to use your jog keys to calibrate to the center of the engraved cross in slot 1.
9. Once successful, select "Save Calibration and Continue.”
10. Step 4 of deck calibration is to use your jog keys to calibrate to the center of the engraved cross in slot 3.
11. Once successful, select "Save Calibration and Continue.”
12. Step 5 of deck calibration is to use your jog keys to calibrate to the center of the engraved cross in slot 7.
13. Once successful, select "Save Calibration and Continue".
14. Remove the tip from the pipette and select "Finish and Restart Robot" to save the new deck calibration data and finish the calibration process.
Please note: The robot will automatically restart itself. This process should not take any longer than 1-2 minutes. During this time, the robot will temporarily lose connection, but will reappear in the robot list after successful restart (please observe that the pipettes home inside the mount to confirm successful robot restart). If the robot does not reappear, click “Try Again” or “Refresh List” in the app, but DO NOT power off the robot. Manually power cycling the robot during this process could terminate calibration and result in issues with robot discovery.
Only if robot discovery continues to fail after 5 minutes of restart should you consider power cycling the robot. In this case, please download the logs under advanced settings and send the api log and serial log to Opentrons technical support via live chat.