You've already calibrated the deck. But before you run a protocol, the Opentrons App will prompt you to do two more kinds of calibration:

  1. Pipette calibration, which accounts for variation in how pipettes are mounted, how tips are attached, and how long tips are.
  2. Labware calibration, which accounts for dimensional and positioning variation in tip racks and labware.

(To learn more, check out How calibration works on the OT-2!)

Calibrate pipettes

From the Protocol tab, select the Calibrate tab or the Proceed to calibrate button:

Then, to begin pipette calibration, select Calibrate tip.

Follow the Opentrons App's instructions. For each pipette:

  1. You'll be prompted to manually attach a tip. (Take care to attach it firmly, and as straight as you can.)
  2. The OT-2 will automatically find the pipette's position. (It will do this by tapping the pipette tip against several tip probe switches.)
  3. You'll be prompted to manually remove the tip.

Once both pipettes have been calibrated, you can move on to labware calibration.

Tip: Although the Opentrons App will prompt you to go through this pipette calibration process every time you upload a protocol, you can sometimes safely skip it.

Calibrate labware

Select Continue to labware setup to move on to labware calibration.

Place the labware onto the deck as shown in the Deck Map.

Caution: Make sure you use the exact labware that the protocol specifies. If you're not sure, you can look the labware up in our Labware Library, where you'll find its dimensions and catalog numbers.

Begin calibrating your labware by selecting Continue moving to tip rack. The pipette will move to the tip rack's A1 position. 

Using the arrow keys or on-screen controls, fine-tune the pipette's position to be exactly centered. Once you're happy with the position, click Pick up tip.

Note: During labware calibration, you shouldn't have to adjust the pipette by more than a few millimeters. Labware calibration is meant for small adjustments. If centering the pipette requires a bigger adjustment, you might not be using the right labware.

The robot will attempt to pick up the tip. If the tip pick up was successful click Yes, Save Calibration . If it was not successful, click No,Try Again  and follow the previous step. 

Continue moving to the rest of the labware using the Move To Next Labware button. Make sure the pipette is flushed and centered with the A1 position in each labware. 

After calibrating all labware, click Return tip and proceed to run.

Tip: Although the Opentrons App will prompt you to go through this process every time you upload a protocol, the App displays any existing calibration data for your labware. Based on this information, you can decide to safely skip labware calibration.

Next steps

Click the Start run button and watch your robot go. Congratulations – you've completed the Guide for Getting Started With Your OT-2!

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