Before you run a protocol, the Opentrons App will prompt you to do two kinds of calibration.

  1. Pipette calibration, which accounts for variation in how pipettes are mounted, how tips are attached, and how long tips are.
  2. Tip rack and labware calibration, which account for dimensional and positioning variation in tip racks and labware.

Pipette Calibration

Click Calibrate tip. The Opentrons App will guide you through pipette calibration.

When manually attaching a tip to a pipette, take care to attach it as straight as possible.

Note: During this process please be advised to not touch/move the pipette or the robot in any way. Doing so will cause the robot to save bad/incorrect data which will affect all calibrations during the run.

Once the pipette has been calibrated, take off the tip and discard. Pipette calibration is now complete!

Labware calibration

Click Continue to labware setup to move on to labware calibration.

Place the labware onto the deck. 

In this protocol there are the following labware: 

  • Tip-rack 200uL - slot 1
  • 96-flat - slot 2
  • Trough- 12 row - slot 5

Begin calibrating your labware by clicking Continue Moving To Tiprack . The gantry will move to the A1  position in the tiprack. 

If need be use the jog key and the increment buttons to center and flush the pipette to the top of the A1 tip. Once you are happy with the position click Pick Up Tip

The robot will attempt to pick up the tip. If the tip pick up was successful click Yes, Save Calibration . If it was not successful, click No,Try Again  and follow the previous step. 

Continue moving to the rest of the labware using the Move To Next Labware button. Make sure the pipette is flushed and centered with the A1 position in each labware. 

After calibrating all labware, click Return tip and proceed to run.

Click the Start run button and watch your robot go. Congratulations – you've completed the Guide for Getting Started With Your OT-2!

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