The robot will perform your Transfer step in aspirate-dispense pairs. The relationship between aspirates and dispenses is always 1:1.
The robot will aspirate from multiple wells before dispensing.
You will have the option to use multi-aspirate when the following conditions are met:
The step is a Many-to-1 Transfer.
Change tip is not set to "Always" or "Per Source Well", which would mean every aspirate requires a new tip.
More than one of your Transfer volume can fit in your pipette's tip.
The robot will dispense into multiple wells per aspirate.
You will have the option to use multi-dispense when the following conditions are met:
The step is a 1-to-Many Transfer.
Change tip is not set to "Always" or "Per Destination Well", which would mean every dispense requires a new tip.
More than one of your Transfer Volume can fit in your pipette's tip, as well as some room for Disposal Volume.
In a multi-dispense sometimes a bit of liquid is lost. The Disposal volume is a buffer of extra liquid to make sure every destination well gets the full Transfer Volume.
After dispenses and before performing its next aspirate the robot will do a Blow Out to clear liquid possibly remaining in the tip.
You have two choices for where to Blow Out:
Trash- if you do not wish to contaminate your source well.
Source Well- if you want to conserve your reagent.
We recommend a minimum of 10% of pipette max for best accuracy but you can change the Disposal Volume to suit your experiment or not use it at all.
Not always one aspirate and multiple dispenses
Occasionally you can create a step where you select the multi-dispense path but the robot will perform a multi-dispense and then one single-transfer.
This happens when there is an odd well out.