MIX WITHIN A TRANSFER
Aspirate- the robot will mix the contents of the well before aspirating the amount it needs to move.
Dispense- the robot will dispense the amount you specified in a well, and then mix what it just dispensed.
THE DIFFERENCE BETWEEN MIX STEP & MIX SETTING
Mix step- Mixes all selected wells one after the other.
Mix setting- Mixes wells before an aspirate or after a dispense.