Below is a trick to get the part of your Serial Dilution that uses a constant transfer volume done in a single Transfer step.

SET UP
Before building this serial dilution Transfer make sure your plate already has the stock solution and diluent laid out. You can begin the protocol with this plate or prepare it using prior steps.

SELECT YOUR ASPIRATE COLUMNS
Select every column you'll aspirate from as part of this section of your serial dilution (this is every column except the last one).

SELECT YOUR DISPENSE COLUMNS
Select every column you'll dispense into as part of this section of your serial dilution (this is every column except the first one).

USE THE MIX SETTING
Use the mix advanced setting to make sure you're always aspirating from a well where the two solutions have been well mixed.

CHECK YOUR WORK
You can hover on rows in the protocol timeline to check where each aspirate and dispense is going. Liquid from A1 should be going to A2, and liquid from A2 to A3, etc.
Use the Final Deck State step to check the results of your dilution in each well.

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