Air gap allows you to aspirate air into the tip after aspirating liquid from a source well. Typically, an air gap is used to prevent dripping of liquid out of the tip.
Air gap can look a little different depending on the Transfer path you have selected.
Single Transfer and Multi-Dispense
The order of operations is as follows:
- Robot will aspirate liquid at the defined tip position, perform any delay or touch tip behaviors selected, then the robot will lift the tip above the well and aspirate air
- Robot will move to the destination well, dispense the air gap volume above the well, then proceed with the rest of the dispense action.
In a multi-aspirate air gap behaves the same way as in a single transfer except for the following difference: In a multi-aspirate air gap happens after every aspirate.