Air gap allows you to aspirate air into the tip after aspirating liquid from a source well. Typically, an air gap is used to prevent dripping of liquid out of the tip.

Air gap can look a little different depending on the Transfer path you have selected.

Aspirate air gap

If the aspirate air gap setting is used, the robot will follow every aspiration of liquid with an air gap. The order of operations per path is as follows:

Single Transfer or Multi-dispense:

  1. Robot will aspirate liquid at the defined tip position, perform any delay or touch tip behaviors selected, then the robot will lift the tip above the well and aspirate air

  2. Robot will move to the destination well, dispense the air gap volume above the well, then proceed with the rest of the dispense action.


In a multi-aspirate air gap behaves the same way as in a single transfer except for the following difference: In a multi-aspirate air gap happens after every aspirate.

Dispense air gap

The dispense air gap setting functions similarly across the three types of paths. It adds an air gap whenever a tip is on its way to the Trash to be disposed of.

Please note: All steps that use a dispense air gap will end by disposing of the last tip used. The next step to use the same pipette will not be allowed to use the “Never” Change Tip setting. If you are seeing an error because you wanted to use the “Never” setting, we recommend you use the “Once at the start of step” setting instead. This will cause the pipette to pick up a tip at the start of the step and keep it for the step’s duration.

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