Air gap allows you to aspirate air into the tip after aspirating liquid from a source well. Typically, an air gap is used to prevent dripping of liquid out of the tip.

Air gap can look a little different depending on the Transfer path you have selected.

Single Transfer and Multi-Dispense

The order of operations is as follows:

  1. Robot will aspirate liquid at the defined tip position, perform any delay or touch tip behaviors selected, then the robot will lift the tip above the well and aspirate air
  2. Robot will move to the destination well, dispense the air gap volume above the well, then proceed with the rest of the dispense action.


In a multi-aspirate air gap behaves the same way as in a single transfer except for the following difference: In a multi-aspirate air gap happens after every aspirate.

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