If you are unsure which robot you are working with, please see our article that highlights the differences between the OT-2 and OT-2R before getting started.

The OT-2R redesigns the pipette mounts and involves a new way to level multi-channel pipettes. The regular method for leveling pipettes still applies to the OT-2R and should be completed first to get the pipette as close to level as possible. If the pipette is still not adequately level afterwards, then proceed with the following instructions.

Note: This method does not completely replace the existing way, where you wiggle the pipette before tightening the 3 pipette attachment screws. It's a fallback in case that way doesn't work.

Updated features in the OT-2R mounts

Increased leveling range and control

With this hardware change, you can adjust new screws in the mount to slightly swing and precisely adjust the pipette so that the pipette nozzles are leveled.

New mount design

The mount has been redesigned with 3 new screws already attached: Top Mount Screw, Bottom Mount Screw, and Leveling Adjustment Screw. The new design is fully compatible with existing Opentrons pipettes (GEN1 and GEN2), except there is now a shorter bottom pipette attachment screw.

Shorter bottom screw length

Before the OT-2R, the bottom pipette attachment screw was 14mm. With the new mount design, it's shortened to 6mm.

Warning: If you use the old (14mm) screw as the bottom pipette attachment screw on an OT-2R mount, the screw will make contact with the plunger of the pipette and prevent it from dropping tips. Putting in the new (6mm) screw will allow the multichannel to work properly. Be sure to confirm you are using the shorter screw while attaching a multi-channel pipette to an OT-2R robot.

⚠️ The 6mm screws are only intended to be used with an OT-2R robot. They will not work on an OT-2.

New Leveling Mechanism

Equipment needed

  • Calibration Block (shipped with GEN2 multi-channel pipettes)

Step 1: Lowering the mount

If you're already following Get started: Attach pipettes and have leveled the attached multi-channel pipette as much as possible, please proceed to Step 2.

1. After connecting your computer to the robot and opening the Opentrons App, navigate to PIPETTES and click CHANGE for the multi-channel pipette you wish to relevel.

2. Follow the directions on the pop-up screen, clearing the deck of labware/modules, and then click the MOVE PIPETTE TO FRONT button.

3. The gantry should home and then move to the front of the robot over deck slot 1 (pipette on right mount) or deck slot 3 (pipette on left mount).

*4. If leveling a GEN2 pipette:

Place the Calibration Block in the deck slot below the pipette, gently pushing the pipette further up to allow for extra space to insert the Block. Keep the pipette at a height where the nozzles are about 2cm above the Calibration Block leveling surface. Make sure that the correct leveling surface is used (P300 - taller level, P20 - shorter level).

**4: If leveling a GEN1 pipette:

Gently and slowly pull the pipette down to about 2cm above the robot's deck.

Step 2: Loosening the Top Mount Screw and Bottom Mount Screw

5. Use the short leg of the 2mm Torx driver to slightly loosen the Top Mount Screw with a ½ counter-clockwise turn.

6. Perform the same ½ counter-clockwise turn for the Bottom Mount Screw, again only slightly loosening it.

Step 3: Leveling the pipette by turning the Leveling Adjustment Screw

7. Insert the long leg of the 2mm Torx driver into the Leveling Adjustment Screw.

8. To swing the pipette towards the front of the robot, turn the Leveling Adjustment Screw clockwise.

9. To swing the pipette towards the back of the robot, turn the Leveling Adjustment Screw counter-clockwise.

Step 4: Confirming the pipette is level

*10. GEN2 pipette: Gently pull the pipette down to the Calibration Block leveling surface and make sure the nozzles all touch flatly across the Block.

**10. GEN1 pipette: Gently pull the pipette down to the deck slot surface and make sure the nozzles all touch flatly across the deck.

In either case, if the difference between channels is less than 0.15mm, it's level enough (0.15mm is the thickness of between one to two sheets of typical printer paper). If the difference is greater than 0.15mm, gently push the pipette back up, off the surface, and turn the Leveling Adjustment Screw accordingly to get the pipette level.

If you're still unable to correct it, your OT-2R might need more adjustment. Contact Opentrons Support for help at or through our live chat.

Step 5: Retightening the Mount Screws

11. Use the short end of the 2mm Torx driver to retighten the Top and Bottom Mount Screws (½ clockwise turn).

Next Steps

  • If you're following the Get Started Guide, you can continue onto calibrating the deck.

  • Otherwise, you can exit out of the Pipette Setup window.

Troubleshooting Notes

1. In the past, the Support team has sent an article out about realigning the Z-stage carriage, which outlined how to level pipettes that were still tilted after adjusting them via the regular method. This should only be done in the most extreme cases after you've gone through this article and spoken to the Support team.

2. If you are using an 8-Channel pipette on an OT-2R, you will want to ensure you are using the correct bottom pipette attachment screw which is shorter than the one used for the OT-2. Otherwise, your 8-Channel pipette will not be able to eject tips.

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