Starting with the Opentrons App

There are a lot of different features on the Opentrons App. This article will provide guidance on what each section uses.

Information panel

When you open your Opentrons App, then you can view the:

  1. Robot name

  2. Server version: Which software the OT-2 is on. This is different than the software version of the app explained below. If they don't match, please contact Opentrons Support at after trying a manual update if issues persist.

  3. Firmware version: Which version the motor controller is on

  4. Supported Protocol API Versions: These are the range of API versions the app will accept min: 2.0, max: 2.10. This won’t affect Protocol Designer

You will see this screen populated once you have successfully connected

Robot controls

  1. Robot Controls > Home All Axes > Home: The Home button will cause the OT-2 to move to the back right corner of the OT-2.

  2. Robot Controls> Restart Robot > Restart: The Restart button will cause your OT-2 to turn off and on again.

  3. Robot Controls>Lights: Will turn on the rail lights for better deck visibility

More Section

When you open the Opentrons app for the first time, you will notice in the lower left corner there is a section that says MORE with three dots above it. Once you select it, you will move to this screen. The home screen of this section is App Software Settings

App software settings

  1. Software Version different from server version (what is on the OT-2), this is what version of the app you have (on your computer).

  2. App update alerts: The OT-2 is set to send you the latest and greatest software update that we have. You can turn this off, but we strongly recommended updating.

  3. Privacy Settings: This just helps us see what errors are occuring with the OT-2s

  4. Advanced Settings> Tip Length Calibration Settings: This is for people that don’t have calibration blocks to use the trash bin instead until the calibration block arrives.

  5. Advanced Settings> Update Channel: Please don’t change this unless you are directed to by Opentrons Support. This is meant for developers like the below functions and is meant for testing purposes.

Custom labware

  1. Here you can add custom labware.

  2. Custom Labware is stored on the computer you are using. If you move computers, you will need to load the custom labware on that computer as well.

Network & System

  1. Manually adding a robot’s IP address

  2. Disable robot catching: Once switched on, the OT-2 will stop recording each OT-2 that has connected to the computer.

  3. Clear Discovered Robot Lists: This is useful if many OT-2’s are rotating on the same computer.

  4. System Information: This is an important step that says “Is there a computer and OT-2 USB connection recognized.” Then it will return the IP6 and IPv4 Address from the USB connection. Technical Support will sometimes ask for this to verify if there is a hardware issue when connecting the OT-2 to the computer.

Other App Features:

Here are some other resources for using the Opentrons app:

  1. Factory Reset

  2. Door Safety Switch

  3. Jupyter Notebook

  4. Understanding the Logs on the OT-2

  5. Using Labware in your protocols

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