This article is about the OT-One.
It's no-longer sold or actively supported by Opentrons, but we've kept this article here to help existing users. Please see our OT-2 Support articles for the most up to date information!
No! As long as you are using the same protocol, you do not have to recalibrate every time you run that protocol. It will remember the calibrations, and the robot will also learn as you continue to run protocols on the robot.