This article is about the OT-One.
It's no-longer sold or actively supported by Opentrons, but we've kept this article here to help existing users. Please see our OT-2 Support articles for the most up to date information!
Instead of adjusting the volume by hand, the robot relies on four user calibrated pipette positions. The calibrations tell the robot where the top of the pipette plunger is, as well as the “soft stop”, blowout and eject tip positions. Once these are set, the robot can calculate how far to depress the plunger to access the entire range of pipette volumes.
Read about how to calibrate and control the plungers on the pipette.