This article is about the OT-One.

It's no-longer sold or actively supported by Opentrons, but we've kept this article here to help existing users. Please see our OT-2 Support articles for the most up to date information!


The OT-One liquid handler uses manual pipettes to transfer liquids. Our robot can push the plunger up and down, similar to how your thumb would. In addition to moving the plunger, the robot’s 'thumbs' also press the pipette’s drop-tip button

The OT-One comes with one single-channel pipette, and the PRO and Hood models come with both a single-channel and an 8-channel pipette. Your pipette(s) will be shipped pre-mounted within your accessory box for easy installation.  Your single-channel pipette can be installed on either the A (center) or B (left) axis, but the 8-channel will only fit on the A axis 


In order to switch your single channel pipette from the B axis to the A axis, the tools you will require are: 

  • Single channel pipette (left)

  • M4 Allen key (right)


Step 1: Loosen the M4 screws enough to be able to slide the single channel pipette off the B axis 

Step 2: Turn the pipette over and unscrew the back screws that are attached to the insert 

Step 3:  Once the acrylic is completely off, flip the acrylic over like so and place on top of the M4 inserts like so 

Step 4: Screw the back screws onto the inserts, and make sure all parts are aligned and flushed 

Make sure the ergonomic tail of the pipette is resting on the back acrylic mounting plate

Step 5: Now that the single channel pipette is mounted correctly to the appropriate hardware, Loosen the two square fastening nuts on the acrylic mounting plate, so they can slide into the extrusion slot

Step 6:  Align the square fastening nuts along the extrusion slot. Slide your pipette upwards into the black stop bar, until the pipette’s ergonomic handle is pressed firmly against the black stopper.

Step 7: Tighten the fastening screws so that your pipette does not move during a protocol run

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