This article is about the OT-One.

It's no-longer sold or actively supported by Opentrons, but we've kept this article here to help existing users. Please see our OT-2 Support articles for the most up to date information!


The OT-One Standard comes with one single-channel pipette, and the PRO and Hood models come with both a single-channel and an 8-channel pipette. Your pipette(s) will be shipped pre-mounted within your accessory box for easy installation.

Due to the differences in plunger heights between the various pipettes, the L bracket must be adjusted on the A and B axis of the robot when switching pipettes. 


In order to adjust the L brackets, you will need:

  • Single or multi channel pipette (left)

  • M4 Allen key (right)


Step 1: Loosen the 3 screws that are on the L bracket with an M4 allen key to allow the L bracket to move up and down the axis 

Step 2: Slide your pipette upwards into the black L bracket, until the pipette’s ergonomic handle is pressed firmly against the black stopper.

Step 3: Reposition the pipette so there is enough room between between the acrylic and plunger

Step 4: Tighten all screws on the pipette with an M4 allen key

Step 5: Tighten the three screws on the L bracket shown in Step 1

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