For our first protocol on the OT2, we will be drawing the Opentrons logo on a 96-well plate!
We will be using two colors, so be sure to find some food dye, or colored liquids you can use. In this tutorial, we’ve chosen to use blue food dye in water, diluted to two different shades.
The procedure is as follows:
Upload protocol > Calibrate > Run
First, download the Opentrons Logo protocol based on the pipette you are using for this run.
Open the OT app, and connect to the robot via wifi or usb
Once the app has been connected to a robot, click the protocol tab. Upload the downloaded Opentrons Logo protocol.
Continue to the calibration screen by either clicking the calibrate tab or the calibrate word under the check mark.
Lets begin with pipette calibration.
Click on the right pipette to begin calibration. All labware must be removed when you are calibrating the pipettes. Confirm the trash has been removed.
The robot will home and come to deck slot 2. Confirm a tip has been attached.
The pipette will now begin the tip probe process.
Note: During this process please be advised to not touch/move the pipette or the robot in any way. Doing so will cause the robot to save bad/incorrect data which will affect all calibrations during the run.
Once the pipette has been calibrated, take off the tip and discard. Pipette calibration is now complete!
Lets move onto labware calibration. Place the labware onto the deck.
In this protocol there are the following labware:
- P300 single channel - right axis
- Tip-rack 200uL - slot 1
- 96-flat - slot 2
- Trough- 12 row - slot 5
Begin calibrating your labware by clicking
Continue Moving To Tiprack . The gantry will move to the
A1 position in the tiprack.
If need be use the jog key and the increment buttons to center and flush the pipette to the top of the
A1 tip. Once you are happy with the position click
Pick Up Tip .
The robot will attempt to pick up the tip. If the tip pick up was successful click
Yes, Save Calibration . If it was not successful, click
No,Try Again and follow the previous step.
Continue moving to the rest of the labware using the
Move To Next Labware button. Make sure the pipette is flushed and centered with the A1 position in each labware.
Once all the labware has been calibrated, click
Return Tip And Proceed To Run. This will take you to the run screen tab of the app. Click
start run . A timer will start along with the command lines from your protocol.
Notice, you can
Cancel Job at anytime.