The care and maintenance of your robot and pipettes is one of the most important routines for working in the lab. A well-cared for pipette will last longer, provide more accurate results, be calibrated more precisely, and reduce the risk of cross-contamination. The following is a comprehensive guide to properly maintaining your robot, cleaning your robot, and cleaning your pipettes. Please use the OT-2 Glossary as a reference for identifying the parts of your robot and pipettes.

Maintaining the Robot

We test our products to 3 years of standard use without the need for tightening screws, oiling bearings, belt tensioning or anything of the sort. No adjustments to this should be needed unless there is some noticeable issue. A user can re-perform deck calibration every ~6-12 months to ensure the alignment of the system remains accurate, should they desire. This deck calibration should compensate for any changes in belt tension, shifts of the machine over time, etc. This is not a mandatory activity, but is optional if you want to ensure everything is still in tip-top-shape.
Note: this time frame may be accelerated depending on frequency of usage.

Materials for Cleaning

Isopropyl Alcohol (70%) or ethyl alcohol (70%)

Some sections of the robot have a preference for one over the other but these two solutions are comparable. 

Bleach (10-15%)

Bleach is not interchangeable with the above solutions

Methods of Cleaning

Wipe

Give material a gentle wipe-down. Make sure there is no liquid dripping and be careful to avoid electronics.

Douse/Spray

These areas are more tolerant of cleaning solution so you can be more aggressive with its application. However still avoid spraying near any electronics.

DO NOT CLEAN

Avoid disrupting these parts. Anything with no explicit cleaning instructions also avoid cleaning. 

Cleaning the Pipettes

Do not disassemble pipettes. Do not douse or spray pipettes because that may damage electronics. When pipette is attached to the robot, power it off, and pull down the pipette to give it a gentle wipe. 

DO NOT CLEAN

Do not clean the electronic components of the pipette

Wipe with Isopropyl Alcohol

Wipe down the following parts of the pipette with isopropyl alcohol: pipette stem, ejector, body, pipette cover, and pipette cap.

Contamination Cleaning

If your pipette becomes contaminated with a known substance, there are specific cleaning steps that must be taken depending on the type of substance. Taking the routine cleaning and maintenance steps outlined above will not be sufficient if your pipette is cross-contaminated. Please contact support if your pipette becomes contaminated for assistance.

For aqueous solutions, rinse the contaminated parts with distilled water or 70 percent ethanol and air dry at 60°F.

For organic solvents, allow the substance to evaporate on its own or immerse the part in a detergent and then air dry.

For radioactive substances, place the pipette in a solution like Decon and then rinse and air dry.

For proteins, rinse the contaminated parts with a detergent, rinse, and air dry. DO NOT use alcohol as it will set the proteins.

For nucleic acids, rinse the contaminated parts in glycine/HCI buffer (pH 2) for 10 minutes, rinse with distilled water, and air dry.

Cleaning the Robot

Robot Gantry

DO NOT CLEAN

Do not douse or spray the gantry because that may damage critical parts. Do not clean Y rails, X rails, pulleys or belts, and anything else that doesn't have explicit cleaning instructions.

Wipe with either Isopropyl Alcohol or Ethyl Alcohol

Wipe down the following parts of the gantry using either isopropyl alcohol or ethyl alcohol: X rail cover, X carriage, and mount cover.

Robot Deck

DO NOT CLEAN

Do not clean tip probe cover or door switch.

Wipe or Douse/Spray with either Isopropyl Alcohol or Ethyl Alcohol

Clean the following parts of the deck using either isopropyl alcohol or ethyl alcohol: removable deck, stationary deck, trash base, and trash cover.

Robot Frame

DO NOT CLEAN

Do not clean the front button or any electronics.

Wipe or Douse/Spray with either Isopropyl Alcohol or Ethyl Alcohol

Clean the following parts of the frame using isopropyl alcohol: black frame, Y rail cover, and silver frame.

With or Douse/Spray with Bleach

Clean the following parts of the frame with ethyl alcohol: acrylic windows and doors.

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